Hydrogen exchange in the formation of dihydroxyacetone phosphate from acyl dihydroxyacetone phosphate in O-alkyl lipid synthesis in Ehrlich ascites tumor cell microsomes.
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منابع مشابه
Hydrogen exchange in the formation of dihydroxyacetone phosphate from acyl dihydroxyacetone phosphate in O-alkyl lipid synthesis in Ehrlich ascites tumor cell microsomes.
We have previously presented evidence that acyl dihydroxyacetone phosphate is converted to O-alkyl dihydroxyacetone phosphate via an endiol intermediate which then accepts a fatty alcohol to form O-alkyl dihydroxyacetone phosphate. We have further proposed that, in the absence of fatty alcohol, the endiol derivative of acyl dihydroxyacetone phosphate reacts with water to form dihydroxyacetone p...
متن کاملAcyl dihydroxyacetone phosphate: precursor of alkyl ethers.
1-0-Alkyl dihydroxyacetone phosphate is biosynthesised in guinea pig liver mitochondria and in rat brain microsomes by direct reaction of a long chain alcohol and acyl dihydroxyacetone phosphate. ATP and Mg* are stimulatory but coenzyme A is not necessary for the reaction. 1-0-Alkyl dihydroxyacetone phosphate (alkyl DHAP)l was shown to be biosynthesized from long chain alcohol and DHAP with ATP...
متن کاملThe acyl dihydroxyacetone phosphate pathway for glycerolipid biosynthesis in mouse liver and Ehrlich ascites tumor cells.
The glycerol portion of lipids may be derived biosynthetically by reduction of dihydroxyacetone phosphate to glycerolphosphate, and then be acylated with fatty acids or, alternatively, dihydroxyacetone phosphate may first be acylated and then reduced to 1-acyl sn glcerol-3-phosphate. Since the former pathway utilizes NADH for reduction of the C-2 carbonyl, while the latter requires NADPH, we we...
متن کاملBiosynthesis of alkyl-ether containing lipid from dihydroxyacetone phosphate.
A lipid, stable to alkaline methanolysis, is formed when liver mitochondria or brain microsomes are incubated with dihydroxyacetone phosphate, long chain alcohol, ATP, CoA, NaF and Mg*. The lipid has been identified as alkyl dihydroxyacetone phosphate. This lipid is reduced by NADPH to 1-alkyl glycerol3-phosphate and is further acylated to form 1-alkyl 2-acyl glycerol-3-phosphate. The ether-con...
متن کاملExclusive localization in peroxisomes of dihydroxyacetone phosphate acyltransferase and alkyl-dihydroxyacetone phosphate synthase in rat liver.
Dihydroxyacetone phosphate acyl transferase (DHAP-AT), alkyl dihydroxyacetone phosphate synthase (alkyl-DHAP-synthase), and glycerol-3-phosphate acyltransferase (GPAT) activities were investigated under optimal assay conditions using highly purified organelle preparations. The data presented clearly indicate that GPAT activity was mainly localized in mitochondria and microsomes, whereas DHAP-AT...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)70132-x